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help verbessert

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jens 2021-01-12 22:13:09 +01:00
parent 852dd86663
commit 72e893c5e7
4 changed files with 7416 additions and 20 deletions
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405nm_2.dat Normal file
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JFShelp.py
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@ -79,7 +79,7 @@ def jfshelpme(win,helpfor):
text.image_create(tk.END,image=photoImg)
text.insert(tk.END, " Calibration\n", 'h1')
text.insert(tk.END, "\nTo calibrate the Instrument we need two laserpointer of different colors und known wavelength.\nFor example a blue [405nm] and red [650nm] one.\n")
text.insert(tk.END, "1) First take two messurements with your device und save the files with the [Save] button under a comprehensible name (e.g. 405nm.data) \n")
text.insert(tk.END, "1) First take two measurements with your device und save the files with the [Save] button under a comprehensible name (e.g. 405nm.data) \n")
text.insert(tk.END, "2) Open the calibration dialog and insert the wavelength in [nm] and afterwards select the respective file. <first peak> stands for the lower and <second peak> for the higher wavelength.\n")
text.insert(tk.END, "3) Now you can use the [Calibrate] button to calibrate the instrument and [Save Config] will the store the configuration.\n")
text.insert(tk.END,"\nAfter this procedure you can switch the [nm] scale on and use other option:\n")
@ -91,8 +91,8 @@ def jfshelpme(win,helpfor):
text.image_create(tk.END,image=photoImg3)
text.insert(tk.END,"\n Baseline\n",'h1')
text.insert(tk.END,"\nIn order to messure the absorption of a compound in the cuvette, you have to messure the intensity of the the light passing through the reference cell.\n")
text.insert(tk.END,"The intensity of the light at a specific wavelength depends on the kind of lightsource. The spectrum of a tungstone lamp is different from a LED etc.\n")
text.insert(tk.END,"\nTo make things easy: the value <threshold for the baseline> cuts off the beginning and the end where the lightsource is not strong enought to give reasonable results ")
text.insert(tk.END,"The intensity of the light at a specific wavelength depends on the kind of light source. The spectrum of a tungstone lamp is different from a LED etc.\n")
text.insert(tk.END,"\nTo make things easy: the value <threshold for the baseline> cuts off the beginning and the end where the light source is not strong enought to give reasonable results ")
text.image_create(tk.END,image=photoImg4)
text.insert(tk.END,"\nFor example xenon-lamp as a source. If the value of the threshold is set to 100 (green), the baseline will start at 380nm and end at 510nm\n")
text.insert(tk.END,"If the value of the threshold is set to 50 (red), the baseline will start at 370nm and end at 695nm. The baseline determines the range of messurement\n")
@ -101,7 +101,7 @@ def jfshelpme(win,helpfor):
text.image_create(tk.END,image=photoImg)
text.insert(tk.END, " Calibration\n", 'h1')
text.insert(tk.END, "\nTo calibrate the Instrument we need two laserpointer of different colors und known wavelength.\nFor example a blue [405nm] and red [650nm] one.\n")
text.insert(tk.END, "1) First take two messurements with your device und save the files with the [Save] button under a comprehensible name (e.g. 405nm.data) \n")
text.insert(tk.END, "1) First take two measurements with your device und save the files with the [Save] button under a comprehensible name (e.g. 405nm.data) \n")
text.insert(tk.END, "2) Open the calibration dialog and insert the wavelength in [nm] and afterwards select the respective file. <first peak> stands for the lower and <second peak> for the higher wavelength.\n")
text.insert(tk.END, "3) Now you can use the [Calibrate] button to calibrate the instrument and [Save Config] will the store the configuration.\n")
text.insert(tk.END,"\nAfter this procedure you can switch the [nm] scale on and use other option:\n")
@ -113,31 +113,31 @@ def jfshelpme(win,helpfor):
text.image_create(tk.END,image=photoImg3)
elif (helpfor == 2):
text.insert(tk.END," Dark spectrum\n",'h1')
text.insert(tk.END,"\nPlease turn off the lightsource and take a messurement with the actual parameter. Afterwards save this spektrum of the dark noise\n")
text.insert(tk.END,"\nPlease turn off the light source and take a messurement with the actual parameter. Afterwards save this spektrum of the dark noise\n")
text.insert(tk.END,"\nClick the checkbutton of [Darkline] to proceed")
elif (helpfor == 3):
text.insert(tk.END," Zero solution \n",'h1')
text.insert(tk.END,"\nIn order to messure the absorbance or transmittance of a sample, you need to messure a zero solution first\n")
text.insert(tk.END,"\n1) Check the darkline checkbutton\n2) Turn on the lightsource and insert a cuvette with a solution without a compound -> \'zero solution\'\n")
text.insert(tk.END,"\n1) Check the darkline checkbutton\n2) Turn on the light source and insert a cuvette with a solution without a compound -> \'zero solution\'\n")
text.insert(tk.END,"3) Save the messurement with [save Base]. This will also cut the range of messurement depending of the lightsoure -> Baseline\n" )
text.insert(tk.END,"4) Click the checkbutton of [Baseline] to proceed\n")
text.insert(tk.END,"Now you can messure the absorbance or transmittance of different compounds by using the radiobuttons \n")
text.insert(tk.END,"\nWith the Dialog [Calibration] you can change the range of the lightsource\n\n")
text.insert(tk.END,"\nWith the Dialog [Calibration] you can change the range of the light source\n\n")
text.insert(tk.END,"\n Baseline\n",'h2')
text.insert(tk.END,"\nIn order to messure the absorption of a compound in the cuvette, you have to messure the intensity of the the light passing through the reference cell.\n")
text.insert(tk.END,"The intensity of the light at a specific wavelength depends on the kind of lightsource. The spectrum of a tungstone lamp is different from a LED etc.\n")
text.insert(tk.END,"\nTo make things easy: the value <threshold for the baseline> cuts off the beginning and the end where the lightsource is not strong enought to give reasonable results ")
text.insert(tk.END,"The intensity of the light at a specific wavelength depends on the kind of light source. The spectrum of a tungstone lamp is different from a LED etc.\n")
text.insert(tk.END,"\nTo make things easy: the value <threshold for the baseline> cuts off the beginning and the end where the light source is not strong enought to give reasonable results ")
text.image_create(tk.END,image=photoImg4)
text.insert(tk.END,"\nFor example xenon-lamp as a source. If the value of the threshold is set to 100 (green), the baseline will start at 380nm and end at 510nm\n")
text.insert(tk.END,"If the value of the threshold is set to 50 (red), the baseline will start at 370nm and end at 695nm. The baseline determines the range of messurement\n")
elif (helpfor == 4):
text.insert(tk.END," Load / Save \n",'h1')
text.insert(tk.END,"\nThese Buttons save und load the photometer data of the absorbance of a compound. The baseline of the lightsource and the dark spectrum of the instrument will also be saved\n")
text.insert(tk.END,"\nThese Buttons save und load the photometer data of the absorbance of a compound. The baseline of the light source and the dark spectrum of the instrument will also be saved\n")
text.insert(tk.END,"\nThis will not work with with simple spetra saves over the [Save] button")
elif (helpfor == 5):
text.image_create(tk.END,image=photoImg3)
text.insert(tk.END,"\nKinetic\n",'h1')
text.insert(tk.END,"\nTo run kinetic messurements with this photometer, make sure that:\n")
text.insert(tk.END,"\nTo run kinetic measurements with this photometer, make sure that:\n")
text.insert(tk.END,"1) The instrument is calibrated\n2) A dark spektrum is taken/saved [save Dark]\n3) A zero solution is messured a saved [save Base].\n\n")
text.insert(tk.END,"If all parameters a ok. Adjust the numbers of repetitions and the time between the messurement in the dialog.\nThe [start Kinetic] Button will start the process.\n")
text.insert(tk.END,"When the messurement is finished the [Math] Dialog appears and the data can be saved and inspected.\n")
@ -146,25 +146,25 @@ def jfshelpme(win,helpfor):
text.insert(tk.END,"\nMath on Kinetics\n",'h1')
text.insert(tk.END,"\nRaw - Absorbanz\n\n",'h2')
text.insert(tk.END,"- Raw: is the raw output of the instrument\n- Raw + Baseline: is raw plus the darkline und the baseline of the lighsource\n")
text.insert(tk.END,"- Transmission: is the relation of the intensity of the light absorpt by the sample and the intensity of the lightsource.\n")
text.insert(tk.END,"- Absorbanz: is the relation of the log(I of lightsource/I of transmitted)\n-- For more information please look for Beer-Lambert law\n")
text.insert(tk.END,"- Transmission: is the relation of the intensity of the light absorpt by the sample and the intensity of the light source.\n")
text.insert(tk.END,"- Absorbanz: is the relation of the log(I of light source/I of transmitted)\n-- For more information please look for Beer-Lambert law\n")
text.insert(tk.END,"\nSlicing\n\n",'h2')
text.insert(tk.END,"If you move the mouse in the left diagram, the coordinats will be displayed in the bottom of the diagram < x=.. and y=.. > x means the point of the nmscale.\n")
text.insert(tk.END,"Clicking with the left mousebutton will select a slice through the curves at the x-value. This slice is displayed in the upper left window. This function works only if the [3 D Print] is not toggled.\n")
text.insert(tk.END,"To check the kinetic order the log(absorbance) is displayed in the lower left window. This can be toggled with the [ln[A] 1/[A]]-button to display 1/[A]\n")
text.insert(tk.END,"\nSelection\n\n",'h2')
text.insert(tk.END,"It is possible to display only parts of the messurements by selecting with the mouse. All other functions should work.\n")
text.insert(tk.END,"It is possible to display only parts of the measurements by selecting with the mouse. All other functions should work.\n")
elif (helpfor == 7):
text.image_create(tk.END,image=photoImg2)
text.insert(tk.END," Methods\n",'h1')
text.insert(tk.END,"\nIn this part of the programm it is possible to determine the concentration of a compound with the spectrometer.\n")
text.insert(tk.END,"1) Try out concentration of the compount,ICG,SH,lightsource etc. that works fine and give an absorbance of approx 1.\nCheck [nm], [Darkline] and [Baseline]\n")
text.insert(tk.END,"1) Try out concentration of the compount, ICG, SH, light source etc. that works fine and give an absorbance of approx 1.\nCheck [nm], [Darkline] and [Baseline]\n")
text.insert(tk.END,"2) Dilute the solution for the standard curve.\nFor example if the last concentration in 30mmol and 5 solution are made. You have to prepare 6mmol,12mmol,18mmol,24mmol and 30mmol.\n")
text.insert(tk.END,"3) Complete the dialog <Edit the method> and [add Method]. The method will appear in the methods window, with a line for each concentration.\n")
text.insert(tk.END,"4) Now insert the cuvette with the specific concentration and select the line.\nThe messurement takes place and the absorbance will be stored\n")
text.insert(tk.END,"4) Now insert the cuvette with the specific concentration and select the line.\nThe measurement takes place and the absorbance will be stored\n")
text.insert(tk.END,"5) If everything is ok, you can save the method and it's values.\n")
text.insert(tk.END,"\nThe fitting curve is determined by numpy's polyfit and the r2_score and displayed.\n")
text.insert(tk.END,"\nAfterwards an unknown concentration of a compound can be determined over the dialog <Do messurements>")
text.insert(tk.END,"\nAfterwards an unknown concentration of a compound can be determined over the dialog <Do measurements>")
### at the end
text.config(state=tk.DISABLED)
top.focus_set()

6
config.ini
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@ -1,7 +1,7 @@
[main]
nm_left = 320.0
nm_right = 811.0
nm_step = 0.13279
nm_left = 334.0
nm_right = 804.0
nm_step = 0.12714
baseline_limit = 100
[methods]