baseline absorbance eingefügt output bandwidth now oriented on Lamp
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d8b0e185a1
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d9a8a0e286
@ -231,6 +231,11 @@ class buildpanel(tk.Frame):
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def darkline():
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if (self.jf.do_save_darkline(config.rxData16)==1):
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self.jfsdark_check.config(state=tk.NORMAL)
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def baseline():
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if (self.jf.get_darkline_checked() == 1):
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base = self.jf.darkData16-config.rxData16
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if (self.jf.do_save_baseline(base)==1):
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self.jfsbase_check.config(state=tk.NORMAL)
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self.jfstitel = tk.Label(self, text=' Photometer ')
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#self.jfstitel.config(font=("Courier",0))
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@ -239,11 +244,14 @@ class buildpanel(tk.Frame):
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self.jfs4cal.grid(row=11,column=4)
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self.jfs4nm_check = tk.Checkbutton(self,text="[nm] scale on/off",variable=self.jf.nm_checked,command=lambda CCDplot=CCDplot: self.updateplot(CCDplot))
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self.jfs4nm_check.grid(row=12,column=4)
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self.jfs4cal = tk.Button(self,text='save Darkline',fg="blue", command= darkline)
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self.jfs4cal.grid(row=13,column=4)
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self.jfsdark = tk.Button(self,text='save Darkline',fg="blue", command= darkline)
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self.jfsdark.grid(row=13,column=4)
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self.jfsdark_check = tk.Checkbutton(self,text="Darkline on/off",variable=self.jf.darkline_checked,state=tk.DISABLED,command=lambda CCDplot=CCDplot: self.updateplot(CCDplot))
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self.jfsdark_check.grid(row=14,column=4)
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self.jfsbase = tk.Button(self,text='save Baseline',fg="blue", command= baseline)
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self.jfsbase.grid(row=15,column=4)
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self.jfsbase_check = tk.Checkbutton(self,text="Baseline on/off",variable=self.jf.baseline_checked,state=tk.DISABLED,command=lambda CCDplot=CCDplot: self.updateplot(CCDplot))
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self.jfsbase_check.grid(row=16,column=4)
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### Callbacks for traces, buttons, etc ###
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def callback(self):
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self.bopen.config(state=tk.DISABLED)
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@ -317,6 +325,33 @@ class buildpanel(tk.Frame):
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def updateplot(self, CCDplot):
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CCDplot.a.clear()
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# Photometer Job Baseline is the Intensity of the Lamp
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# baseline start and baseline end depends on the spektral range of the Lamp
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# the actuel input is lower due to the absorption of the liquid
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if (self.jf.get_baseline_checked()==1):
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config.pltBaseData16 = self.jf.baseData16
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config.pltData16 = self.jf.darkData16 - config.rxData16
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for i in range(0,3694):
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if (config.pltBaseData16[i] == 0):
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config.pltData16[i] = 1
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else:
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config.pltData16[i] = (config.pltData16[i] / config.pltBaseData16[i])
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config.pltData16 = 100*config.pltData16
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up = np.max(config.pltData16) + 50
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if (self.jf.get_nm_checked()==1):
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CCDplot.a.plot(self.jf.get_nm_scale(),config.pltData16)
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CCDplot.a.axis([self.jf.nm_left,self.jf.nm_right,-10,up])
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CCDplot.a.set_xlim([self.jf.nm_left+self.jf.baseline_start*self.jf.nm_step, self.jf.nm_left+self.jf.baseline_end*self.jf.nm_step])
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CCDplot.a.set_xlabel(" [nm] ")
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else:
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CCDplot.a.plot(config.pltData16)
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CCDplot.a.axis([0,3694,-10,up])
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CCDplot.a.set_xlim([self.jf.baseline_start, self.jf.baseline_end])
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CCDplot.a.set_xlabel("Pixelnumber")
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CCDplot.a.set_ylabel("Intensity")
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else:
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#This subtracts the ADC-pixel from ADC-dark
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if (config.datainvert==1):
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if (self.jf.get_darkline_checked()==1):
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@ -329,7 +364,8 @@ class buildpanel(tk.Frame):
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#print(config.offset)
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for i in range (1847):
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config.pltData16[2*i] = config.pltData16[2*i] - config.offset
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CCDplot.a.clear()
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#CCDplot.a.clear()
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#plot intensities
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if (config.datainvert == 1):
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## make max visible
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38
JFSphoto.py
38
JFSphoto.py
@ -29,6 +29,12 @@ class Jfsphoto (object):
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self.darkData16 = np.zeros(3694, np.float32)
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self.darkline_checked = tk.IntVar()
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self.darkline_checked.set(0)
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########## baseline of the lightsource
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self.baseData16 = np.zeros(3694, np.float32)
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self.baseline_checked = tk.IntVar()
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self.baseline_checked.set(0)
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self.baseline_start=0
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self.baseline_end=0
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def do_calibrate(self):
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win = tk.Toplevel()
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@ -85,6 +91,7 @@ class Jfsphoto (object):
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self.button.pack()
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self.mroot.mainloop()
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############ Darkline
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def do_save_darkline(self,dark):
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x = np.min(dark)
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if (x < 3700):
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@ -97,6 +104,37 @@ class Jfsphoto (object):
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def get_darkline_checked(self):
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return self.darkline_checked.get()
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############ Baseline depends on the capacity of the light source block spektralrange if the intensity is to low
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def do_save_baseline(self,base):
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### no margin at all
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doIt = True
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#### no left margin
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left = True
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x = np.max(base)
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if (x < 500):
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self.do_msg(" This is not a Baseine \n Is the Lightsouce switch on ? ")
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return 0
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else:
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for i in range(0,3694):
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if (base[i] < 200) :
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if doIt:
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if left:
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self.baseline_start = i
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else:
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self.baseline_end = i
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doIt = False
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self.baseData16[i] = 0
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else:
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if (base[i] > 300):
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left = False
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self.baseData16[i] = base[i]*1.0
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print(self.baseline_start," ",self.baseline_end)
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return 1
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def get_baseline_checked(self):
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return self.baseline_checked.get()
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############ Calibration and Load/Save Configuration Part 2
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def checkit(self):
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self.nm = int(self.tnm.get())
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self.nmi = int(self.tnmi.get())
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@ -10,7 +10,7 @@ SHperiod = np.uint32(200)
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ICGperiod = np.uint32(100000)
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AVGn = np.uint8([0,1])
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MCLK = 2000000
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SHsent = np.uint32(200)
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SHsent = np.uint32(50)
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ICGsent = np.uint32(100000)
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stopsignal = 0
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@ -18,6 +18,8 @@ stopsignal = 0
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rxData8 = np.zeros(7388, np.uint8)
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rxData16 = np.zeros(3694, np.uint16)
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pltData16 = np.zeros(3694, np.float32)
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pltBaseData16 = np.zeros(3694, np.float32)
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#Arrays for data to transmit
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txsh = np.uint8([0,0,0,0])
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